Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Non-thermal plasma-activated methionine-containing Ringer’s solution enhances cytotoxicity by increasing oxidative stress

doi: 10.3164/jcbn.25-241

Figure Lengend Snippet: Effects of NTP-irradiation of Met-related compounds on cell viability. (A) Met-containing dipeptides. MDA-MB-231 cells were treated with PAA, Met/PAA, plasma-activated Met-Gly-containing AR, or plasma-activated Gly-Met-containing AR for 24 ‍h. The cell viability was measured by MTT assay. The results are shown as mean ± SD ( n = 4). ** p <0.01 (vs unirradiated AR). ## p <0.01 (vs irradiated AR, 10%). (B) Methyl-cysteine. MDA-MB-231 cells were treated with Met/PAA or plasma-activated S -methyl- l -cysteine-containing AR (Me-Cys/PAA) for 24 ‍h. The cell viability was measured by MTT assay. The results are shown as mean ± SD ( n = 4). ** p <0.01 (vs PAA alone).

Article Snippet: Methionine and dl -methionine sulfoxide were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). l -Methionylglycine (Met-Gly) and Glycyl- l -methionine (Gly-Met) were purchased from Tokyo Chemical Industry (Tokyo, Japan).

Techniques: Irradiation, Clinical Proteomics, MTT Assay